Statistical noise is a feature of every quantitative PCR (qPCR) curve. In principle, two different forms of noise can be encountered: (i) the dispersion of fluorescence signals about their true values ...

Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to reference genes. It is critical to select ...

The MIQE guidelines and papers such as the one discussed here can be applied to avoid pitfalls. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is everywhere. Its ubiquity, ...

The polymerase chain reaction (PCR) has radically transformed biological science, allowing sophisticated analysis of genes and the genome. Revolutionizing the study of DNA, PCR is often hailed as one ...

Considering the importance of gene expression studies for understanding the biology of glioma stem cells (GSCs), we aimed to identify the reliable reference genes in GSCs that were derived from the ...

Biogazelle presents qbasePLUS 2, a major upgrade of the most powerful, flexible, and user-friendly real-time PCR data-analysis software. qbasePLUS is based on the peer reviewed Ghent University ...