Measuring Enzyme Kinetic Parameters with the ITC Universal Enzyme Assay Method Enzymes are important in drug discovery and development since they constitute approximately one-half of all known drug ...

This article describes a simple method to determine the optimum pH for the activity of one enzyme in a coupled enzyme assay. Decoupling of the two-step reaction is achieved by heat denaturing the ...

Traditional enzymatic assays are often technologically limited by plate readers with narrow detection ranges that only capture either faint or intense signals, forcing researchers into laborious and ...

The development of new therapeutics targeting protein kinases is crucial in the fight against diseases like cancer.

During the last 50 years, an abundance of data has been gathered on the function of lipids as a source of energy, as building blocks, and as signaling molecules in living systems. Lipid-signaling ...

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